Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5694Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 8000ng/L | 1.73 | 1.854 | 1.806 |
| 1.978 | |||
| 4000ng/L | 1.076 | 0.97 | 0.923 |
| 0.865 | |||
| 2000ng/L | 0.53 | 0.522 | 0.474 |
| 0.514 | |||
| 1000ng/L | 0.246 | 0.271 | 0.224 |
| 0.297 | |||
| 500ng/L | 0.126 | 0.1 | 0.053 |
| 0.075 | |||
| 0ng/L | 0.052 | 0.048 | 0 |
| 0.043 |
Precision
We measured random samples of E5694Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 1475.13 | 63.5 | 4.3 |
| Intra-Assay | 2 | 18 | 4599.59 | 221.34 | 4.81 |
| Intra-Assay | 3 | 18 | 5399.08 | 120.48 | 2.23 |
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