Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5722Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 3200ng/L | 2.44 | 2.521 | 2.483 |
| 2.602 | |||
| 1600ng/L | 1.371 | 1.48 | 1.442 |
| 1.589 | |||
| 800ng/L | 0.754 | 0.825 | 0.787 |
| 0.896 | |||
| 400ng/L | 0.489 | 0.472 | 0.434 |
| 0.456 | |||
| 200ng/L | 0.27 | 0.243 | 0.205 |
| 0.216 | |||
| 0ng/L | 0.044 | 0.038 | 0 |
| 0.032 |
Precision
We measured random samples of E5722Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 384.55 | 18.12 | 4.71 |
| Intra-Assay | 2 | 18 | 1443.82 | 41.8 | 2.89 |
| Intra-Assay | 3 | 18 | 893.53 | 53.26 | 5.96 |
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