Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5756Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 24ng/mL | 2.152 | 2.023 | 1.983 |
| 1.894 | |||
| 12ng/mL | 1.051 | 1.022 | 0.981 |
| 0.992 | |||
| 6ng/mL | 0.491 | 0.508 | 0.467 |
| 0.525 | |||
| 3ng/mL | 0.296 | 0.292 | 0.252 |
| 0.289 | |||
| 1.5ng/mL | 0.118 | 0.107 | 0.066 |
| 0.095 | |||
| 0ng/mL | 0.051 | 0.04 | 0 |
| 0.03 |
Precision
We measured random samples of E5756Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 19.78 | 0.54 | 2.72 |
| Intra-Assay | 2 | 18 | 6.08 | 0.32 | 5.27 |
| Intra-Assay | 3 | 18 | 14.89 | 0.57 | 3.8 |
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