Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5762Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 48ng/mL | 2.299 | 2.2 | 2.131 |
| 2.101 | |||
| 24ng/mL | 1.463 | 1.396 | 1.327 |
| 1.33 | |||
| 12ng/mL | 0.818 | 0.805 | 0.736 |
| 0.793 | |||
| 6ng/mL | 0.55 | 0.549 | 0.48 |
| 0.549 | |||
| 3ng/mL | 0.251 | 0.277 | 0.208 |
| 0.303 | |||
| 0ng/mL | 0.073 | 0.069 | 0 |
| 0.065 |
Precision
We measured random samples of E5762Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 8.33 | 0.44 | 5.27 |
| Intra-Assay | 2 | 18 | 20.45 | 1.12 | 5.46 |
| Intra-Assay | 3 | 18 | 31.46 | 1.05 | 3.34 |
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