Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5809Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 240ng/L | 2.049 | 2.175 | 2.126 |
| 2.302 | |||
| 120ng/L | 1.554 | 1.527 | 1.478 |
| 1.501 | |||
| 60ng/L | 0.785 | 0.866 | 0.817 |
| 0.948 | |||
| 30ng/L | 0.485 | 0.513 | 0.463 |
| 0.54 | |||
| 15ng/L | 0.229 | 0.238 | 0.189 |
| 0.248 | |||
| 0ng/L | 0.048 | 0.05 | 0 |
| 0.052 |
Precision
We measured random samples of E5809Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 152.91 | 6.89 | 4.5 |
| Intra-Assay | 2 | 18 | 37.36 | 2.45 | 6.55 |
| Intra-Assay | 3 | 18 | 65.44 | 3.19 | 4.87 |
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