Human Fibroblast Growth Factor 1, FGF1 ELISA Kit

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Home > Product > ELISA Kit > Human Fibroblast Growth Factor 1, FGF1 ELISA Kit

Human Fibroblast Growth Factor 1, FGF1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.85ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.85ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Fibroblast Growth Factor 1

Code

E3828Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.85ng/L

Detection range

20-6000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Acts as a ligand for FGFR1 and integrins. Binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation via sequential autophosphorylation on tyrosine residues which act as docking sites for interacting proteins, leading to the activation of several signaling cascades. Binds to integrin ITGAV:ITGB3. Its binding to integrin, subsequent ternary complex formation with integrin and FGFR1, and the recruitment of PTPN11 to the complex are essential for FGF1 signaling. Induces the phosphorylation and activation of FGFR1, FRS2, MAPK3/ERK1, MAPK1/ERK2 and AKT1 (PubMed: 18441324, PubMed: 20422052). Can induce angiogenesis (PubMed: 23469107).

UniProt accession

P05230

MASS（Da）

17,460

GeneID

2246

Synonyms

Acidic fibroblast growth factor;aFGF;ECGF;Endothelial cell growth factor;FGF 1;FGF1;FGF-1;Fibroblast growth factor 1;HBGF-1;Heparin-binding growth factor 1

Gene names

FGF1

Research area

Cardiovascular

Target protein

FGF1

Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Protocol data

These standard curves of E3828Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4000ng/L	2.234	2.236	2.18
	2.239
2000ng/L	1.378	1.441	1.384
	1.504
1000ng/L	0.879	0.894	0.837
	0.908
500ng/L	0.524	0.521	0.464
	0.518
250ng/L	0.298	0.269	0.212
	0.24
0ng/L	0.043	0.057	0
	0.07

Precision

We measured random samples of E3828Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%

Protocols

> Protocol Booklet

References

Customer reviews and Q&As

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Associated products

Datasheet

Size

Code

Price

48T

E3828Hu

$320.00
96T

E3828Hu

$458.00

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Lead time: Within two weeks

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