Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5818Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 80mU/mL | 2.069 | 2.107 | 2.054 |
| 2.145 | |||
| 40mU/mL | 1.695 | 1.768 | 1.715 |
| 1.841 | |||
| 20mU/mL | 1.299 | 1.218 | 1.165 |
| 1.138 | |||
| 10mU/mL | 0.712 | 0.716 | 0.663 |
| 0.721 | |||
| 5mU/mL | 0.425 | 0.417 | 0.364 |
| 0.409 | |||
| 0mU/mL | 0.063 | 0.053 | 0 |
| 0.043 |
Precision
We measured random samples of E5818Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 19.71 | 1.36 | 6.9 |
| Intra-Assay | 2 | 18 | 38.33 | 2.59 | 6.76 |
| Intra-Assay | 3 | 18 | 8.95 | 0.42 | 4.65 |
Protocols
References
Customer reviews and Q&As
