Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

UniProt accession

MASS（Da）

GeneID

Synonyms

Gene names

Research area

Target protein

Components	Quantity
Pre-coated ELISA plate	12 wells * 8 detachable strips
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

These standard curves of E5821Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
480ng/mL	1.828	1.975	1.926
	2.122
240ng/mL	0.902	0.998	0.949
	1.094
120ng/mL	0.605	0.556	0.507
	0.508
60ng/mL	0.303	0.252	0.203
	0.201
30ng/mL	0.161	0.139	0.09
	0.116
0ng/mL	0.041	0.049	0
	0.057

We measured random samples of E5821Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	270.67	12.71	4.7
Intra-Assay	2	18	313.5	8.99	2.87
Intra-Assay	3	18	89.77	3.98	4.43

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