Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5821Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 480ng/mL | 1.828 | 1.975 | 1.926 |
| 2.122 | |||
| 240ng/mL | 0.902 | 0.998 | 0.949 |
| 1.094 | |||
| 120ng/mL | 0.605 | 0.556 | 0.507 |
| 0.508 | |||
| 60ng/mL | 0.303 | 0.252 | 0.203 |
| 0.201 | |||
| 30ng/mL | 0.161 | 0.139 | 0.09 |
| 0.116 | |||
| 0ng/mL | 0.041 | 0.049 | 0 |
| 0.057 |
Precision
We measured random samples of E5821Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 270.67 | 12.71 | 4.7 |
| Intra-Assay | 2 | 18 | 313.5 | 8.99 | 2.87 |
| Intra-Assay | 3 | 18 | 89.77 | 3.98 | 4.43 |
Protocols
References
Customer reviews and Q&As
