Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5858Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1600ng/L | 2.156 | 2.147 | 2.116 |
| 2.137 | |||
| 800ng/L | 1.311 | 1.272 | 1.241 |
| 1.232 | |||
| 400ng/L | 0.72 | 0.701 | 0.67 |
| 0.681 | |||
| 200ng/L | 0.386 | 0.388 | 0.357 |
| 0.391 | |||
| 100ng/L | 0.201 | 0.171 | 0.14 |
| 0.14 | |||
| 0ng/L | 0.036 | 0.031 | 0 |
| 0.026 |
Precision
We measured random samples of E5858Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 599.24 | 35.02 | 5.84 |
| Intra-Assay | 2 | 18 | 1055.14 | 41.58 | 3.94 |
| Intra-Assay | 3 | 18 | 413.37 | 24.19 | 5.85 |
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