Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5874Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 2400ng/L | 2.073 | 2.151 | 2.127 |
| 2.229 | |||
| 1200ng/L | 1.531 | 1.455 | 1.43 |
| 1.379 | |||
| 600ng/L | 0.77 | 0.658 | 0.633 |
| 0.546 | |||
| 300ng/L | 0.326 | 0.384 | 0.359 |
| 0.442 | |||
| 150ng/L | 0.173 | 0.189 | 0.164 |
| 0.204 | |||
| 0ng/L | 0.025 | 0.025 | 0 |
| 0.025 |
Precision
We measured random samples of E5874Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 1066.96 | 38.46 | 3.6 |
| Intra-Assay | 2 | 18 | 980.93 | 41.54 | 4.23 |
| Intra-Assay | 3 | 18 | 334.43 | 14.21 | 4.25 |
Protocols
References
Customer reviews and Q&As
