Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5901Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 3200ng/L | 2.063 | 2.106 | 2.076 |
| 2.149 | |||
| 1600ng/L | 0.931 | 0.985 | 0.956 |
| 1.039 | |||
| 800ng/L | 0.593 | 0.553 | 0.524 |
| 0.513 | |||
| 400ng/L | 0.297 | 0.292 | 0.263 |
| 0.287 | |||
| 200ng/L | 0.15 | 0.157 | 0.128 |
| 0.164 | |||
| 0ng/L | 0.03 | 0.029 | 0 |
| 0.029 |
Precision
We measured random samples of E5901Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 1997.76 | 44.26 | 2.22 |
| Intra-Assay | 2 | 18 | 927.07 | 50.4 | 5.44 |
| Intra-Assay | 3 | 18 | 1763.89 | 82.14 | 4.66 |
Protocols
References
Customer reviews and Q&As
