Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5903Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1600ng/L | 1.87 | 1.896 | 1.848 |
| 1.923 | |||
| 800ng/L | 0.928 | 1.027 | 0.978 |
| 1.125 | |||
| 400ng/L | 0.431 | 0.515 | 0.466 |
| 0.599 | |||
| 200ng/L | 0.156 | 0.191 | 0.143 |
| 0.227 | |||
| 100ng/L | 0.111 | 0.096 | 0.047 |
| 0.082 | |||
| 0ng/L | 0.055 | 0.049 | 0 |
| 0.043 |
Precision
We measured random samples of E5903Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 1149.07 | 32.37 | 2.82 |
| Intra-Assay | 2 | 18 | 639.2 | 23.21 | 3.63 |
| Intra-Assay | 3 | 18 | 430.18 | 18.48 | 4.3 |
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