Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5905Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 12ng/mL | 2.317 | 2.306 | 2.276 |
| 2.296 | |||
| 6ng/mL | 1.522 | 1.593 | 1.563 |
| 1.664 | |||
| 3ng/mL | 1.15 | 1.076 | 1.046 |
| 1.001 | |||
| 1.5ng/mL | 0.693 | 0.676 | 0.646 |
| 0.659 | |||
| 0.75ng/mL | 0.456 | 0.441 | 0.411 |
| 0.425 | |||
| 0ng/mL | 0.034 | 0.03 | 0 |
| 0.026 |
Precision
We measured random samples of E5905Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 1.23 | 0.08 | 6.49 |
| Intra-Assay | 2 | 18 | 6.2 | 0.27 | 4.43 |
| Intra-Assay | 3 | 18 | 2.26 | 0.16 | 7.2 |
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