Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5956Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1200ng/L | 2.094 | 2.263 | 2.215 |
| 2.432 | |||
| 600ng/L | 1.308 | 1.332 | 1.284 |
| 1.355 | |||
| 300ng/L | 0.798 | 0.816 | 0.769 |
| 0.835 | |||
| 150ng/L | 0.543 | 0.522 | 0.475 |
| 0.501 | |||
| 75ng/L | 0.303 | 0.286 | 0.238 |
| 0.269 | |||
| 0ng/L | 0.053 | 0.048 | 0 |
| 0.043 |
Precision
We measured random samples of E5956Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 919.5 | 33.1 | 3.6 |
| Intra-Assay | 2 | 18 | 283.95 | 16.4 | 5.78 |
| Intra-Assay | 3 | 18 | 210.17 | 13.25 | 6.3 |
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