Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5960Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 2400ng/L | 1.869 | 1.919 | 1.876 |
| 1.969 | |||
| 1200ng/L | 0.949 | 0.988 | 0.945 |
| 1.026 | |||
| 600ng/L | 0.54 | 0.551 | 0.507 |
| 0.561 | |||
| 300ng/L | 0.254 | 0.272 | 0.229 |
| 0.29 | |||
| 150ng/L | 0.157 | 0.145 | 0.102 |
| 0.134 | |||
| 0ng/L | 0.026 | 0.043 | 0 |
| 0.06 |
Precision
We measured random samples of E5960Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 844.05 | 54.82 | 6.5 |
| Intra-Assay | 2 | 18 | 933.64 | 47.09 | 5.04 |
| Intra-Assay | 3 | 18 | 1798.6 | 46.43 | 2.58 |
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