Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E6043Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1200ng/L | 2.196 | 2.141 | 2.091 |
| 2.087 | |||
| 600ng/L | 1.881 | 1.838 | 1.787 |
| 1.794 | |||
| 300ng/L | 1.221 | 1.266 | 1.216 |
| 1.312 | |||
| 150ng/L | 0.689 | 0.666 | 0.616 |
| 0.644 | |||
| 75ng/L | 0.435 | 0.417 | 0.366 |
| 0.398 | |||
| 0ng/L | 0.058 | 0.051 | 0 |
| 0.043 |
Precision
We measured random samples of E6043Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 91.69 | 3.8 | 4.14 |
| Intra-Assay | 2 | 18 | 415.15 | 22.22 | 5.35 |
| Intra-Assay | 3 | 18 | 179.54 | 8.76 | 4.88 |
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