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Home > Product > ELISA Kit > Human Radiation-inducible Immediate-Early Gene Iex-1, IER3 ELISA Kit

Human Radiation-inducible Immediate-Early Gene Iex-1, IER3 ELISA Kit

Human Radiation-inducible Immediate-Early Gene Iex-1, IER3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.18ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.18ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Radiation-inducible immediate-early gene IEX-1

Code

E6053Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.18ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

May play a role in the ERK signaling pathway by inhibiting the dephosphorylation of ERK by phosphatase PP2A-PPP2R5C holoenzyme. Acts also as an ERK downstream effector mediating survival. As a member of the NUPR1/RELB/IER3 survival pathway, may provide pancreatic ductal adenocarcinoma with remarkable resistance to cell stress, such as starvation or gemcitabine treatment.

UniProt accession

MASS(Da)

16,903

GeneID

8870

Synonyms

DIF2;DIF-2;Differentiation-dependent gene 2 protein;GLY96;IER3;IEX1;IEX-1;Immediate early protein GLY96;Immediate early response 3 protein;PACAP-responsive gene 1 protein;PRG1;Protein DIF-2;Protein PRG1;Radiation-inducible immediate-early gene IEX-1

Gene names

IER3

Research area

Cell Biology; Stem Cells

Target protein

IER3

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6053Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.288 2.309 2.265
2.329
1200ng/L 1.286 1.297 1.253
1.309
600ng/L 0.853 0.883 0.839
0.913
300ng/L 0.498 0.467 0.423
0.437
150ng/L 0.224 0.226 0.182
0.229
0ng/L 0.041 0.044 0
0.047

Precision

We measured random samples of E6053Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 589.09 31.43 5.33
Intra-Assay 2 18 1699.19 56.56 3.33
Intra-Assay 3 18 237.5 16.55 6.97

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E6053Hu

    $320.00

  • 96T

    E6053Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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