Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Human Rap Gguanine Nucleotide Exchange Factor 4, RAPGEF4 ELISA Kit

Human Rap Gguanine Nucleotide Exchange Factor 4, RAPGEF4 ELISA Kit

Human Rap Gguanine Nucleotide Exchange Factor 4, RAPGEF4 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.67ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 7.67ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Rap guanine nucleotide exchange factor 4

Code

E6056Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.67ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2 (By similarity).

UniProt accession

MASS(Da)

115,522

GeneID

11069

Synonyms

CAMP-GEFII;cAMP-regulated guanine nucleotide exchange factor II;CGEF2;EPAC;EPAC 2;EPAC2;Exchange factor directly activated by cAMP 2;Exchange protein directly activated by cAMP 2;Nbla00496;Rap guanine nucleotide exchange factor 4;RAPGEF4

Gene names

RAPGEF4

Research area

Signal Transduction

Target protein

RAPGEF4

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6056Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.235 2.134 2.064
2.033
800ng/L 1.425 1.416 1.346
1.407
400ng/L 0.827 0.839 0.769
0.851
200ng/L 0.504 0.458 0.388
0.412
100ng/L 0.272 0.294 0.223
0.316
0ng/L 0.071 0.07 0
0.069

Precision

We measured random samples of E6056Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 507.52 25.36 5
Intra-Assay 2 18 871.86 29.03 3.33
Intra-Assay 3 18 178.83 9.66 5.4

Protocols

References

Customer reviews and Q&As

No reviews
No reviews

Associated products

  • Size

    Code

    Price

  • 48T

    E6056Hu

    $320.00

  • 96T

    E6056Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up