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Home > Product > ELISA Kit > Human Repulsive Guidance Molecule A, RGMA ELISA Kit

Human Repulsive Guidance Molecule A, RGMA ELISA Kit

Human Repulsive Guidance Molecule A, RGMA ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.024ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.024ng/mL

Detection range: 0.05-20ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Repulsive guidance molecule A

Code

E6078Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.024ng/mL

Detection range

0.05-20ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8.

UniProt accession

MASS(Da)

49,347

GeneID

56963

Synonyms

Repulsive guidance molecule A;RGM;RGM domain family member A;RGMA

Gene names

RGMA

Research area

Others

Target protein

RGMA

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6078Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/mL 1.857 1.855 1.812
1.852
6ng/mL 1.156 1.03 0.987
0.904
3ng/mL 0.551 0.537 0.494
0.524
1.5ng/mL 0.268 0.27 0.227
0.273
0.75ng/mL 0.086 0.1 0.057
0.114
0ng/mL 0.031 0.043 0
0.056

Precision

We measured random samples of E6078Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 7.13 0.32 4.49
Intra-Assay 2 18 7.78 0.19 2.49
Intra-Assay 3 18 3.82 0.18 4.76

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E6078Hu

    $320.00

  • 96T

    E6078Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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