Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E6094Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 2400ng/L | 2.063 | 2.032 | 1.99 |
| 2.002 | |||
| 1200ng/L | 1.439 | 1.413 | 1.371 |
| 1.387 | |||
| 600ng/L | 0.58 | 0.654 | 0.612 |
| 0.729 | |||
| 300ng/L | 0.315 | 0.33 | 0.288 |
| 0.344 | |||
| 150ng/L | 0.12 | 0.159 | 0.117 |
| 0.198 | |||
| 0ng/L | 0.04 | 0.042 | 0 |
| 0.045 |
Precision
We measured random samples of E6094Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 1645.24 | 88.28 | 5.37 |
| Intra-Assay | 2 | 18 | 337.18 | 14.41 | 4.27 |
| Intra-Assay | 3 | 18 | 799.81 | 35.07 | 4.38 |
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