Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E6099Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 640ng/L | 2.336 | 2.501 | 2.458 |
| 2.666 | |||
| 320ng/L | 1.431 | 1.391 | 1.348 |
| 1.35 | |||
| 160ng/L | 0.898 | 0.841 | 0.798 |
| 0.784 | |||
| 80ng/L | 0.415 | 0.437 | 0.394 |
| 0.459 | |||
| 40ng/L | 0.222 | 0.235 | 0.193 |
| 0.249 | |||
| 0ng/L | 0.044 | 0.043 | 0 |
| 0.041 |
Precision
We measured random samples of E6099Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 291.36 | 8.94 | 3.07 |
| Intra-Assay | 2 | 18 | 90.22 | 4.99 | 5.53 |
| Intra-Assay | 3 | 18 | 187.96 | 8.73 | 4.64 |
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