Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E6113Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 3200ng/L | 2.529 | 2.498 | 2.437 |
| 2.468 | |||
| 1600ng/L | 1.456 | 1.449 | 1.387 |
| 1.441 | |||
| 800ng/L | 0.741 | 0.798 | 0.736 |
| 0.854 | |||
| 400ng/L | 0.396 | 0.443 | 0.381 |
| 0.489 | |||
| 200ng/L | 0.242 | 0.238 | 0.176 |
| 0.235 | |||
| 0ng/L | 0.058 | 0.062 | 0 |
| 0.066 |
Precision
We measured random samples of E6113Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 2012.4 | 76.12 | 3.78 |
| Intra-Assay | 2 | 18 | 1065.58 | 61.66 | 5.79 |
| Intra-Assay | 3 | 18 | 422.6 | 19.32 | 4.57 |
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