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Home > Product > ELISA Kit > Human Sarcoplasmic Endoplasmic Reticulum Calcium Atpase 1, ATP2A1 ELISA Kit

Human Sarcoplasmic Endoplasmic Reticulum Calcium Atpase 1, ATP2A1 ELISA Kit

Human Sarcoplasmic Endoplasmic Reticulum Calcium Atpase 1, ATP2A1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 4.89ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 4.89ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Sarcoplasmic/endoplasmic reticulum calcium ATPase 1

Code

E6118Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

4.89ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Key regulator of striated muscle performance by acting as the major Ca2+ ATPase responsible for the reuptake of cytosolic Ca2+ into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen (By similarity). Contributes to calcium sequestration involved in muscular excitation/contraction (PubMed:10914677).

UniProt accession

MASS(Da)

110,252

GeneID

487

Synonyms

ATP2A;ATP2A1;Calcium pump 1;Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform;Endoplasmic reticulum class 1/2 Ca(2+ ATPase;Sarcoplasmic/endoplasmic reticulum calcium ATPase 1;SERCA1;SR Ca(2+-ATPase 1

Gene names

ATP2A1

Research area

Tags & Cell Markers

Target protein

ATP2A1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6118Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.061 2.102 2.054
2.143
600ng/L 1.439 1.468 1.421
1.498
300ng/L 0.922 0.938 0.89
0.954
150ng/L 0.471 0.492 0.445
0.514
75ng/L 0.306 0.302 0.254
0.297
0ng/L 0.061 0.048 0
0.034

Precision

We measured random samples of E6118Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 179.01 9.02 5.04
Intra-Assay 2 18 891.21 46.97 5.27
Intra-Assay 3 18 356.18 17.99 5.05

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E6118Hu

    $320.00

  • 96T

    E6118Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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