Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E6141Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 12ng/mL | 2.159 | 2.149 | 2.094 |
| 2.139 | |||
| 6ng/mL | 1.278 | 1.33 | 1.275 |
| 1.382 | |||
| 3ng/mL | 0.699 | 0.728 | 0.673 |
| 0.758 | |||
| 1.5ng/mL | 0.514 | 0.494 | 0.44 |
| 0.475 | |||
| 0.75ng/mL | 0.292 | 0.3 | 0.245 |
| 0.307 | |||
| 0ng/mL | 0.051 | 0.055 | 0 |
| 0.059 |
Precision
We measured random samples of E6141Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 5.26 | 0.3 | 5.69 |
| Intra-Assay | 2 | 18 | 8.19 | 0.31 | 3.74 |
| Intra-Assay | 3 | 18 | 2.64 | 0.18 | 6.83 |
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