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Home > Product > ELISA Kit > Human Serine Threonine-protein Kinase Endoribonuclease Ire1, ERN1 ELISA Kit

Human Serine Threonine-protein Kinase Endoribonuclease Ire1, ERN1 ELISA Kit

Human Serine Threonine-protein Kinase Endoribonuclease Ire1, ERN1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.76ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 7.76ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Serine/threonine-protein kinase/endoribonuclease IRE1

Code

E6150Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.76ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR) (PubMed:11779464, PubMed:11175748, PubMed:12637535, PubMed:9637683, PubMed:21317875, PubMed:28128204). In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP (PubMed:21317875). Accumulation of misfolded proteins in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity (PubMed:21317875). The endoribonuclease activity is specific for XBP1 mRNA and excises 26 nucleotides from XBP1 mRNA (PubMed:11779464, PubMed:24508390, PubMed:21317875). The resulting spliced transcript of XBP1 encodes a transcriptional activator protein that up-regulates expression of UPR target genes (PubMed:11779464, PubMed:24508390, PubMed:21317875). Acts as an upstream signal for ER stress-induced GORASP2-mediated unconventional (ER/Golgi-independent) trafficking of CFTR to cell membrane by modulating the expression and localization of SEC16A (PubMed:21884936, PubMed:28067262).

UniProt accession

MASS(Da)

109,735

GeneID

2081

Synonyms

Endoplasmic reticulum-to-nucleus signaling 1;Endoribonuclease;ERN1;hIRE1p;Inositol-requiring protein 1;IRE1;IRE1a;IRE1a [Includes: Serine/threonine-protein kinase;Ire1-alpha;IRE1P;Serine/threonine-protein kinase/endoribonuclease IRE1

Gene names

ERN1

Research area

Signal Transduction

Target protein

ERN1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6150Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.271 2.203 2.147
2.135
800ng/L 1.491 1.522 1.466
1.552
400ng/L 0.749 0.846 0.79
0.943
200ng/L 0.489 0.495 0.439
0.5
100ng/L 0.25 0.254 0.198
0.258
0ng/L 0.055 0.056 0
0.057

Precision

We measured random samples of E6150Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 699.47 23.09 3.3
Intra-Assay 2 18 439.04 14.12 3.22
Intra-Assay 3 18 207.63 10.89 5.25

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E6150Hu

    $320.00

  • 96T

    E6150Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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