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Home > Product > ELISA Kit > Human Serine Threonine-protein Phosphatase 2A 65 kDa Regulatory Subunit A Alpha Isoform, PPP2R1A ELISA Kit

Human Serine Threonine-protein Phosphatase 2A 65 kDa Regulatory Subunit A Alpha Isoform, PPP2R1A ELISA Kit

Human Serine Threonine-protein Phosphatase 2A 65 kDa Regulatory Subunit A Alpha Isoform, PPP2R1A ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.02ng/L

Detection range: 20-3500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.02ng/L

Detection range: 20-3500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform

Code

E6152Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.02ng/L

Detection range

20-3500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Upon interaction with GNA12 promotes dephosphorylation of microtubule associated protein TAU/MAPT (PubMed:15525651). Required for proper chromosome segregation and for centromeric localization of SGO1 in mitosis (PubMed:16580887).

UniProt accession

MASS(Da)

65,309

GeneID

5518

Synonyms

Medium tumor antigen-associated 61 kDa protein;MRD36;PP2A subunit A isoform PR65-alpha;PP2A subunit A isoform R1-alpha;PP2AA;PP2AAALPHA;PP2A-Aalpha;PPP2R1A;PR65A;Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform

Gene names

PPP2R1A

Research area

Others

Target protein

PPP2R1A

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6152Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2000ng/L 2.145 2.092 2.043
2.039
1000ng/L 1.761 1.854 1.805
1.947
500ng/L 1.299 1.237 1.188
1.176
250ng/L 0.701 0.724 0.675
0.746
125ng/L 0.441 0.399 0.35
0.356
0ng/L 0.054 0.049 0
0.044

Precision

We measured random samples of E6152Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 549.84 22.52 4.1
Intra-Assay 2 18 156.23 7.24 4.63
Intra-Assay 3 18 339.65 18.08 5.32

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E6152Hu

    $320.00

  • 96T

    E6152Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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