Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E6158Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 48ng/mL | 2.371 | 2.387 | 2.334 |
| 2.403 | |||
| 24ng/mL | 1.479 | 1.425 | 1.372 |
| 1.372 | |||
| 12ng/mL | 0.849 | 0.815 | 0.762 |
| 0.782 | |||
| 6ng/mL | 0.457 | 0.476 | 0.423 |
| 0.495 | |||
| 3ng/mL | 0.245 | 0.266 | 0.212 |
| 0.287 | |||
| 0ng/mL | 0.062 | 0.054 | 0 |
| 0.045 |
Precision
We measured random samples of E6158Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 19.49 | 1.05 | 5.41 |
| Intra-Assay | 2 | 18 | 29.09 | 1.14 | 3.93 |
| Intra-Assay | 3 | 18 | 5.37 | 0.28 | 5.14 |
Customer reviews and Q&As
Associated products