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Home > Product > ELISA Kit > Human Small Nuclear Ribonucleoprotein Sm D1, SNRPD1 ELISA Kit

Human Small Nuclear Ribonucleoprotein Sm D1, SNRPD1 ELISA Kit

Human Small Nuclear Ribonucleoprotein Sm D1, SNRPD1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.88ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.88ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Small nuclear ribonucleoprotein Sm D1

Code

E6181Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.88ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Plays role in pre-mRNA splicing as core component of the SMN-Sm complex that mediates spliceosomal snRNP assembly and as component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome (PubMed:11991638, PubMed:18984161, PubMed:19325628, PubMed:23333303, PubMed:25555158, PubMed:26912367, PubMed:28502770, PubMed:28781166, PubMed:28076346). Component of both the pre-catalytic spliceosome B complex and activated spliceosome C complexes (PubMed:11991638, PubMed:26912367, PubMed:28502770, PubMed:28781166, PubMed:28076346). Is also a component of the minor U12 spliceosome (PubMed:15146077). May act as a charged protein scaffold to promote snRNP assembly or strengthen snRNP-snRNP interactions through non-specific electrostatic contacts with RNA (Probable).

UniProt accession

MASS(Da)

13,282

GeneID

6632

Synonyms

HsT2456;Small nuclear ribonucleoprotein Sm D1;Sm-D autoantigen;SMD1;Sm-D1;snRNP core protein D1;SNRPD;SNRPD1

Gene names

SNRPD1

Research area

Others

Target protein

SNRPD1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6181Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 1.948 1.981 1.934
2.015
1200ng/L 1.067 1.075 1.027
1.083
600ng/L 0.433 0.466 0.418
0.499
300ng/L 0.17 0.203 0.155
0.237
150ng/L 0.145 0.139 0.092
0.134
0ng/L 0.054 0.048 0
0.042

Precision

We measured random samples of E6181Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 2020.46 69.33 3.43
Intra-Assay 2 18 893.02 30.68 3.44
Intra-Assay 3 18 629.93 19.69 3.13

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E6181Hu

    $320.00

  • 96T

    E6181Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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