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Home > Product > ELISA Kit > Human Supervillin, SVIL ELISA Kit

Human Supervillin, SVIL ELISA Kit

Human Supervillin, SVIL ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.99ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 7.99ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Supervillin

Code

E6233Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.99ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Forms a high-affinity link between the actin cytoskeleton and the membrane. Is among the first costameric proteins to assemble during myogenesis and it contributes to myogenic membrane structure and differentiation (PubMed:12711699). Appears to be involved in myosin II assembly. May modulate myosin II regulation through MLCK during cell spreading, an initial step in cell migration. May play a role in invadopodial function (PubMed:19109420). May be involved in modulation of focal adhesions. Supervillin-mediated down-regulation of focal adhesions involves binding to TRIP6. Plays a role in cytokinesis through KIF14 interaction (By similarity).

UniProt accession

MASS(Da)

247,746

GeneID

6840

Synonyms

Archvillin;MFM10;p205/p250;Supervillin;SVIL

Gene names

SVIL

Research area

Signal Transduction

Target protein

SVIL

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6233Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 1.721 1.811 1.757
1.901
800ng/L 0.894 0.917 0.863
0.94
400ng/L 0.501 0.569 0.515
0.638
200ng/L 0.204 0.214 0.16
0.225
100ng/L 0.073 0.11 0.056
0.147
0ng/L 0.046 0.054 0
0.062

Precision

We measured random samples of E6233Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 986.77 51.63 5.23
Intra-Assay 2 18 1458.74 34.91 2.39
Intra-Assay 3 18 296.3 13.5 4.56

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E6233Hu

    $320.00

  • 96T

    E6233Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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