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Home > Product > ELISA Kit > Human Sterol 26-hydroxylase, Mitochondrial, CYP27A1 ELISA Kit

Human Sterol 26-hydroxylase, Mitochondrial, CYP27A1 ELISA Kit

Human Sterol 26-hydroxylase, Mitochondrial, CYP27A1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 4.89ng/L

Detection range: 10-4000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 4.89ng/L

Detection range: 10-4000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Sterol 26-Hydroxylase,Mitochondrial

Code

E3869Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

4.89ng/L

Detection range

10-4000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Cytochrome P450 monooxygenase that catalyzes regio- and stereospecific hydroxylation of cholesterol and its derivatives. Hydroxylates (with R stereochemistry) the terminal methyl group of cholesterol side-chain in a three step reaction to yield at first a C26 alcohol, then a C26 aldehyde and finally a C26 acid (PubMed: 9660774, PubMed: 12077124, PubMed: 21411718, PubMed: 28190002). Regulates cholesterol homeostasis by catalyzing the conversion of excess cholesterol to bile acids via both the 'neutral' (classic) and the 'acid' (alternative) pathways (PubMed: 9660774, PubMed: 1708392, PubMed: 11412116, PubMed: 2019602, PubMed: 7915755, PubMed: 9186905, PubMed: 9790667). May also regulate cholesterol homeostasis via generation of active oxysterols, which act as ligands for NR1H2 and NR1H3 nuclear receptors, modulating the transcription of genes involved in lipid metabolism (PubMed: 9660774, PubMed: 12077124). Plays a role in cholestanol metabolism in the cerebellum. Similarly to cholesterol, hydroxylates cholestanol and may facilitate sterol diffusion through the blood-brain barrier to the systemic circulation for further degradation (PubMed: 28190002). Also hydroxylates retinal 7-ketocholesterol, a noxious oxysterol with pro-inflammatory and pro-apoptotic effects, and may play a role in its elimination from the retinal pigment epithelium (PubMed: 21411718). May play a redundant role in vitamin D biosynthesis. Catalyzes 25-hydroxylation of vitamin D3 that is required for its conversion to a functionally active form (PubMed: 15465040).

UniProt accession

MASS(Da)

60,235

GeneID

1593

Synonyms

5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 26-hydroxylase;CYP27A1;Cytochrome P450 27;Cytochrome P-450C27/25;Sterol 26-hydroxylase, mitochondrial;Sterol 27-hydroxylase;Vitamin D(3 25-hydroxylase

Gene names

CYP27A1

Research area

Cardiovascular

Target protein

CYP27A1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3869Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.293 2.339 2.311
2.385
1200ng/L 1.294 1.356 1.328
1.417
600ng/L 0.762 0.757 0.729
0.752
300ng/L 0.348 0.328 0.3
0.309
150ng/L 0.117 0.118 0.09
0.119
0ng/L 0.027 0.028 0
0.029

Precision

We measured random samples of E3869Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1281.5 38.15 3
Intra-Assay 2 18 307.4 11.85 3.9
Intra-Assay 3 18 985 47.01 4.8

Protocols

References

Customer reviews and Q&As

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Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

Add to Cart

Lead time: Within one week

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