Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E6236Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 24ng/mL | 2.032 | 2.097 | 2.045 |
| 2.162 | |||
| 12ng/mL | 1.366 | 1.443 | 1.391 |
| 1.52 | |||
| 6ng/mL | 0.786 | 0.788 | 0.736 |
| 0.79 | |||
| 3ng/mL | 0.505 | 0.497 | 0.445 |
| 0.489 | |||
| 1.5ng/mL | 0.293 | 0.286 | 0.234 |
| 0.279 | |||
| 0ng/mL | 0.036 | 0.052 | 0 |
| 0.068 |
Precision
We measured random samples of E6236Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 10.2 | 0.59 | 5.73 |
| Intra-Assay | 2 | 18 | 13.6 | 0.68 | 4.99 |
| Intra-Assay | 3 | 18 | 4.42 | 0.26 | 5.86 |
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