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Home > Product > ELISA Kit > Human Transcription Factor 3B 50 kDa Subunit, BRF2 ELISA Kit

Human Transcription Factor 3B 50 kDa Subunit, BRF2 ELISA Kit

Human Transcription Factor 3B 50 kDa Subunit, BRF2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.75ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 7.75ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Transcription factor 3B 50 kDa subunit

Code

E6289Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.75ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites (PubMed:11040218, PubMed:11121026, PubMed:11564744, PubMed:26638071). Contributes to the regulation of gene expression; functions as activator in the absence of oxidative stress (PubMed:26638071). Down-regulates expression of target genes in response to oxidative stress (PubMed:26638071). Overexpression protects cells against apoptosis in response to oxidative stress (PubMed:26638071).

UniProt accession

MASS(Da)

46,533

GeneID

55290

Synonyms

B-related factor 2;BRF2;BRF-2;BRFU;hBRFU;hTFIIIB50;TFIIIB50;Transcription factor IIIB 50 kDa subunit

Gene names

BRF2

Research area

Epigenetics and Nuclear Signaling

Target protein

BRF2

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6289Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.072 2.056 2.018
2.041
800ng/L 1.154 1.217 1.178
1.279
400ng/L 0.768 0.713 0.675
0.658
200ng/L 0.44 0.443 0.405
0.447
100ng/L 0.206 0.211 0.173
0.216
0ng/L 0.031 0.038 0
0.046

Precision

We measured random samples of E6289Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 682.52 41.13 6.03
Intra-Assay 2 18 1068 38.63 3.62
Intra-Assay 3 18 336.82 18.58 5.52

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E6289Hu

    $320.00

  • 96T

    E6289Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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