Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E6314Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1600ng/L | 2.215 | 2.145 | 2.109 |
| 2.076 | |||
| 800ng/L | 1.64 | 1.661 | 1.625 |
| 1.683 | |||
| 400ng/L | 1.091 | 1.136 | 1.099 |
| 1.181 | |||
| 200ng/L | 0.7 | 0.735 | 0.699 |
| 0.771 | |||
| 100ng/L | 0.424 | 0.457 | 0.42 |
| 0.489 | |||
| 0ng/L | 0.045 | 0.037 | 0 |
| 0.028 |
Precision
We measured random samples of E6314Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 386.14 | 30.72 | 7.95 |
| Intra-Assay | 2 | 18 | 480.7 | 24.84 | 5.17 |
| Intra-Assay | 3 | 18 | 100.78 | 7.37 | 7.31 |
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