Human Tripartite Motif-containing Protein 72, TRIM72 ELISA Kit

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Home > Product > ELISA Kit > Human Tripartite Motif-containing Protein 72, TRIM72 ELISA Kit

Human Tripartite Motif-containing Protein 72, TRIM72 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.31ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 7.31ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Tripartite motif-containing protein 72

Code

E6322Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.31ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca2+-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles (By similarity).

UniProt accession

Q6ZMU5

MASS（Da）

52,731

GeneID

493829

Synonyms

MG53;Mitsugumin-53;TRIM72;Tripartite motif-containing protein 72

Gene names

TRIM72

Research area

Signal Transduction; Cell Biology

Target protein

TRIM72

Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Protocol data

These standard curves of E6322Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.179	2.228	2.182
	2.277
800ng/L	1.341	1.348	1.302
	1.355
400ng/L	0.852	0.843	0.798
	0.835
200ng/L	0.463	0.489	0.443
	0.516
100ng/L	0.264	0.262	0.216
	0.261
0ng/L	0.053	0.046	0
	0.038

Precision

We measured random samples of E6322Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1027.4	38.84	3.78
Intra-Assay	2	18	504.99	31.85	6.31
Intra-Assay	3	18	283.06	19.65	6.94

Protocols

> Protocol Booklet

References

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Associated products

Datasheet

Size

Code

Price

48T

E6322Hu

$320.00
96T

E6322Hu

$458.00

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Lead time: Within two weeks

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