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Home > Product > ELISA Kit > Human Tryptophan 5-hydroxylase 2, TPH2 ELISA Kit

Human Tryptophan 5-hydroxylase 2, TPH2 ELISA Kit

Human Tryptophan 5-hydroxylase 2, TPH2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.71ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.71ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Tryptophan 5-hydroxylase 2

Code

E6331Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.71ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Tryptophan hydroxylase 2 (TPH2) is an isozyme of tryptophan hydroxylase found in vertebrates. In humans, TPH2 is primarily expressed in the serotonergic neurons of the brain, with the highest expression in the raphe nucleus of the midbrain. Until the discovery of TPH2 in 2003, serotonin levels in the central nervous system were believed to be regulated by serotonin synthesis in peripheral tissues, in which tryptophan hydroxylase is the dominant form. Tryptophan hydroxylase (TPH; EC 1.14.16.4) is the rate-limiting enzyme in the synthesis of serotonin (5-hydroxytryptamine, or 5HT). 5HT is causally involved in numerous central nervous activities, and it has several functions in peripheral tissues, including the maintenance of vascular tone and gut motility.

UniProt accession

MASS(Da)

56,057

GeneID

121278

Synonyms

ADHD7;Neuronal tryptophan hydroxylase;NTPH;TPH2;Tryptophan 5-hydroxylase 2;Tryptophan 5-monooxygenase 2

Gene names

TPH2

Research area

Others

Target protein

TPH2

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6331Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.202 2.182 2.142
2.161
1200ng/L 1.925 1.842 1.803
1.759
600ng/L 1.121 1.177 1.137
1.233
300ng/L 0.664 0.679 0.639
0.694
150ng/L 0.442 0.432 0.393
0.423
0ng/L 0.03 0.039 0
0.049

Precision

We measured random samples of E6331Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 961.76 59.37 6.17
Intra-Assay 2 18 567.99 40.75 7.17
Intra-Assay 3 18 160.2 8.53 5.33

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E6331Hu

    $320.00

  • 96T

    E6331Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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