Human Tyrosyl-DNA Phosphodiesterase 1, TDP1 ELISA Kit

Product

OneStep ELISA kit
Primacu ELISA kit
Antibodies
Biochemicals
Recombinant proteins

Service

Custom ELISA assay services
Bulk quote

Research area

Blood coagulation
Metabolism
Cancer
Microbiology
Cardiovascular
Neuroscience
Cell biology
Signal transduction
Developmental biology
Stem cells
Epigenetics and nuclear signaling
Tags & cell markers
Immunology

Resources

Protocols
FAQs
Product brochure
Promotion
Troubleshooting guides
About ELISAs

About us

Company profile
Worldwide distributors
Manufacturing service

Home > Product > ELISA Kit > Human Tyrosyl-DNA Phosphodiesterase 1, TDP1 ELISA Kit

Human Tyrosyl-DNA Phosphodiesterase 1, TDP1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.85ng/L

Detection range: 20-7000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.85ng/L

Detection range: 20-7000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Tyrosyl-DNA Phosphodiesterase 1

Code

E6368Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.85ng/L

Detection range

20-7000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. Hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. Acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. Has low 3'exonuclease activity and can remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. Has no exonuclease activity towards DNA or RNA with a 3'phosphate.

UniProt accession

Q9NUW8

MASS（Da）

68,420

GeneID

55775

Synonyms

TDP1;Tyr-DNA phosphodiesterase 1;Tyrosyl-DNA phosphodiesterase 1

Gene names

TDP1

Research area

Others

Target protein

TDP1

Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Protocol data

These standard curves of E6368Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4000ng/L	2.066	2.08	2.046
	2.095
2000ng/L	0.922	0.916	0.882
	0.91
1000ng/L	0.572	0.534	0.5
	0.496
500ng/L	0.223	0.193	0.159
	0.162
250ng/L	0.101	0.09	0.056
	0.079
0ng/L	0.025	0.034	0
	0.043

Precision

We measured random samples of E6368Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2630.76	61.21	2.33
Intra-Assay	2	18	1845.08	68.86	3.73
Intra-Assay	3	18	1111.77	49.14	4.42

Protocols

> Protocol Booklet

References

Customer reviews and Q&As

No reviews

Associated products

Datasheet

Size

Code

Price

48T

E6368Hu

$320.00
96T

E6368Hu

$458.00

Add to Cart

Lead time: Within two weeks

Custom quote | Bulk request

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.