Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E6383Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1200ng/L | 1.948 | 1.988 | 1.933 |
| 2.029 | |||
| 600ng/L | 1.087 | 1.034 | 0.979 |
| 0.98 | |||
| 300ng/L | 0.533 | 0.568 | 0.513 |
| 0.604 | |||
| 150ng/L | 0.271 | 0.272 | 0.217 |
| 0.273 | |||
| 75ng/L | 0.147 | 0.144 | 0.09 |
| 0.142 | |||
| 0ng/L | 0.045 | 0.055 | 0 |
| 0.065 |
Precision
We measured random samples of E6383Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 579.14 | 23.28 | 4.02 |
| Intra-Assay | 2 | 18 | 1042.9 | 31.78 | 3.05 |
| Intra-Assay | 3 | 18 | 207.58 | 8.94 | 4.31 |
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