Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E6384Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 800ng/L | 2.222 | 2.226 | 2.183 |
| 2.23 | |||
| 400ng/L | 1.177 | 1.22 | 1.178 |
| 1.264 | |||
| 200ng/L | 0.788 | 0.785 | 0.742 |
| 0.782 | |||
| 100ng/L | 0.384 | 0.447 | 0.405 |
| 0.51 | |||
| 50ng/L | 0.236 | 0.248 | 0.205 |
| 0.259 | |||
| 0ng/L | 0.058 | 0.043 | 0 |
| 0.028 |
Precision
We measured random samples of E6384Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 635.48 | 22.11 | 3.48 |
| Intra-Assay | 2 | 18 | 232.29 | 15.84 | 6.82 |
| Intra-Assay | 3 | 18 | 132.6 | 9.73 | 7.34 |
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