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Home > Product > ELISA Kit > Human Wd Repeat- and Fyve Domain-containing Protein 4, WDFY4 ELISA Kit

Human Wd Repeat- and Fyve Domain-containing Protein 4, WDFY4 ELISA Kit

Human Wd Repeat- and Fyve Domain-containing Protein 4, WDFY4 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.39ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 5.39ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

WD repeat- and FYVE domain-containing protein 4

Code

E6408Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.39ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Plays a critical role in the regulation of cDC1-mediated cross-presentation of viral and tumor antigens in dendritic cells. Mechanistically, acts near the plasma membrane and interacts with endosomal membranes to promote endosomal-to-cytosol antigen trafficking. Plays also a role in B-cell survival through regulation of autophagy.

UniProt accession

MASS(Da)

353,610

GeneID

57705

Synonyms

C10orf64;WD repeat- and FYVE domain-containing protein 4;WDFY4

Gene names

WDFY4

Research area

Cell Biology

Target protein

WDFY4

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6408Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.507 2.431 2.375
2.354
600ng/L 1.393 1.391 1.336
1.389
300ng/L 0.839 0.85 0.795
0.861
150ng/L 0.374 0.392 0.337
0.41
75ng/L 0.243 0.231 0.176
0.22
0ng/L 0.066 0.055 0
0.044

Precision

We measured random samples of E6408Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 201.37 10.8 5.37
Intra-Assay 2 18 466.88 26.25 5.62
Intra-Assay 3 18 595.48 22.62 3.8

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E6408Hu

    $320.00

  • 96T

    E6408Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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