Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E6411Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1200ng/L | 1.736 | 1.875 | 1.83 |
| 2.014 | |||
| 600ng/L | 1.083 | 0.989 | 0.944 |
| 0.895 | |||
| 300ng/L | 0.62 | 0.56 | 0.516 |
| 0.5 | |||
| 150ng/L | 0.145 | 0.198 | 0.153 |
| 0.25 | |||
| 75ng/L | 0.114 | 0.111 | 0.067 |
| 0.109 | |||
| 0ng/L | 0.029 | 0.044 | 0 |
| 0.059 |
Precision
We measured random samples of E6411Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 889.32 | 27.94 | 3.14 |
| Intra-Assay | 2 | 18 | 700.21 | 30.51 | 4.36 |
| Intra-Assay | 3 | 18 | 270.48 | 11.99 | 4.43 |
Customer reviews and Q&As
Associated products