Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E6457Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 40ng/ml | 2.349 | 2.291 | 2.246 |
| 2.233 | |||
| 20ng/ml | 1.599 | 1.659 | 1.614 |
| 1.718 | |||
| 10ng/ml | 1.064 | 1.055 | 1.01 |
| 1.045 | |||
| 5ng/ml | 0.784 | 0.744 | 0.699 |
| 0.704 | |||
| 2.5ng/ml | 0.434 | 0.449 | 0.404 |
| 0.464 | |||
| 0ng/ml | 0.046 | 0.045 | 0 |
| 0.044 |
Precision
We measured random samples of E6457Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 4.81 | 0.39 | 8.12 |
| Intra-Assay | 2 | 18 | 20.9 | 1.27 | 6.07 |
| Intra-Assay | 3 | 18 | 11.78 | 1.03 | 8.76 |
Protocols
References
Customer reviews and Q&As
Associated products
