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Home > Product > ELISA Kit > Human Rna Polymerase Ii Subunit A C-terminal Domain Phosphatase, CTDP1 ELISA Kit

Human Rna Polymerase Ii Subunit A C-terminal Domain Phosphatase, CTDP1 ELISA Kit

Human Rna Polymerase Ii Subunit A C-terminal Domain Phosphatase, CTDP1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.32ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.32ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

RNA polymerase II subunit A C-terminal domain phosphatase

Code

E6507Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.32ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Processively dephosphorylates 'Ser-2' and 'Ser-5' of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit. This promotes the activity of RNA polymerase II. Plays a role in the exit from mitosis by dephosphorylating crucial mitotic substrates (USP44, CDC20 and WEE1) that are required for M-phase-promoting factor (MPF)/CDK1 inactivation.

UniProt accession

MASS(Da)

104,399

GeneID

9150

Synonyms

CCFDN;CTDP1;FCP1;RNA polymerase II subunit A C-terminal domain phosphatase;TFIIF-associating CTD phosphatase

Gene names

CTDP1

Research area

Signal Transduction; Epigenetics and Nuclear Signaling

Target protein

CTDP1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6507Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.197 2.151 2.103
2.104
1200ng/L 1.336 1.384 1.336
1.432
600ng/L 0.769 0.831 0.782
0.892
300ng/L 0.549 0.509 0.46
0.468
150ng/L 0.275 0.254 0.206
0.232
0ng/L 0.048 0.048 0
0.048

Precision

We measured random samples of E6507Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1726.61 63.41 3.67
Intra-Assay 2 18 429.17 25.73 6
Intra-Assay 3 18 865.94 44.9 5.19

Protocols

References

Customer reviews and Q&As

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Associated products

Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

Add to Cart

Lead time: Within one week

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