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Home > Product > ELISA Kit > Human Cholesterol Side-chain Cleavage Enzyme, CYP11A1 ELISA Kit

Human Cholesterol Side-chain Cleavage Enzyme, CYP11A1 ELISA Kit

Human Cholesterol Side-chain Cleavage Enzyme, CYP11A1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 8.07ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 8.07ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Cholesterol side-chain cleavage enzyme

Code

E6517Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

8.07ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

A cytochrome P450 monooxygenase that catalyzes the side-chain hydroxylation and cleavage of cholesterol to pregnenolone, the precursor of most steroid hormones (PubMed:21636783). Catalyzes three sequential oxidation reactions of cholesterol, namely the hydroxylation at C22 followed with the hydroxylation at C20 to yield 20R,22R-hydroxycholesterol that is further cleaved between C20 and C22 to yield the C21-steroid pregnenolone and 4-methylpentanal (PubMed:21636783). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin) (PubMed:21636783).

UniProt accession

MASS(Da)

60,102

GeneID

1583

Synonyms

Cholesterol desmolase;Cholesterol side-chain cleavage enzyme, mitochondrial;CYP11A;CYP11A1;CYPXIA1;Cytochrome P450 11A1;Cytochrome P450(scc;P450SCC

Gene names

CYP11A1

Research area

Cardiovascular; Signal Transduction; Cancer; Metabolism

Target protein

CYP11A1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6517Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.384 2.408 2.375
2.433
800ng/L 1.228 1.209 1.175
1.191
400ng/L 0.618 0.687 0.653
0.757
200ng/L 0.405 0.369 0.335
0.334
100ng/L 0.186 0.174 0.14
0.161
0ng/L 0.02 0.034 0
0.048

Precision

We measured random samples of E6517Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 359.23 17.08 4.76
Intra-Assay 2 18 703.94 37.35 5.31
Intra-Assay 3 18 1112.5 34.5 3.1

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E6517Hu

    $320.00

  • 96T

    E6517Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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