Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E6526Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1200ng/L | 2.143 | 2.137 | 2.094 |
| 2.131 | |||
| 600ng/L | 1.358 | 1.333 | 1.29 |
| 1.308 | |||
| 300ng/L | 0.629 | 0.658 | 0.615 |
| 0.687 | |||
| 150ng/L | 0.289 | 0.329 | 0.286 |
| 0.37 | |||
| 75ng/L | 0.203 | 0.181 | 0.138 |
| 0.159 | |||
| 0ng/L | 0.046 | 0.043 | 0 |
| 0.039 |
Precision
We measured random samples of E6526Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 855.25 | 32.51 | 3.8 |
| Intra-Assay | 2 | 18 | 518.13 | 17.76 | 3.43 |
| Intra-Assay | 3 | 18 | 175.67 | 8.1 | 4.61 |
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