Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

UniProt accession

MASS（Da）

GeneID

Synonyms

Gene names

Research area

Target protein

Components	Quantity
Pre-coated ELISA plate	12 wells * 8 detachable strips
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

These standard curves of E6549Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.307	2.184	2.136
	2.061
6ng/ml	1.242	1.227	1.179
	1.211
3ng/ml	0.781	0.752	0.704
	0.723
1.5ng/ml	0.533	0.477	0.43
	0.422
0.75ng/ml	0.228	0.205	0.158
	0.183
0ng/ml	0.039	0.048	0
	0.057

We measured random samples of E6549Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	6.01	0.22	3.62
Intra-Assay	2	18	3.89	0.25	6.32
Intra-Assay	3	18	2.8	0.18	6.46