Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E6550Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 12ng/ml | 2.104 | 2.223 | 2.161 |
| 2.342 | |||
| 6ng/ml | 1.372 | 1.302 | 1.24 |
| 1.233 | |||
| 3ng/ml | 0.697 | 0.783 | 0.72 |
| 0.868 | |||
| 1.5ng/ml | 0.497 | 0.47 | 0.408 |
| 0.443 | |||
| 0.75ng/ml | 0.234 | 0.234 | 0.172 |
| 0.234 | |||
| 0ng/ml | 0.063 | 0.062 | 0 |
| 0.062 |
Precision
We measured random samples of E6550Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 3.92 | 0.23 | 5.74 |
| Intra-Assay | 2 | 18 | 9.24 | 0.3 | 3.22 |
| Intra-Assay | 3 | 18 | 1.87 | 0.13 | 6.87 |
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