Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E6596Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 480ng/mL | 2.068 | 2.191 | 2.154 |
| 2.314 | |||
| 240ng/mL | 1.201 | 1.286 | 1.249 |
| 1.371 | |||
| 120ng/mL | 0.692 | 0.752 | 0.715 |
| 0.812 | |||
| 60ng/mL | 0.393 | 0.387 | 0.35 |
| 0.382 | |||
| 30ng/mL | 0.25 | 0.226 | 0.189 |
| 0.202 | |||
| 0ng/mL | 0.025 | 0.037 | 0 |
| 0.049 |
Precision
We measured random samples of E6596Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 213.12 | 9.43 | 4.42 |
| Intra-Assay | 2 | 18 | 264.71 | 6.32 | 2.39 |
| Intra-Assay | 3 | 18 | 63.24 | 3.97 | 6.28 |
Protocols
References
Customer reviews and Q&As
