Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E6611Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1600ng/L | 2.137 | 2.2 | 2.173 |
| 2.263 | |||
| 800ng/L | 1.476 | 1.514 | 1.486 |
| 1.552 | |||
| 400ng/L | 0.584 | 0.583 | 0.555 |
| 0.581 | |||
| 200ng/L | 0.343 | 0.358 | 0.33 |
| 0.372 | |||
| 100ng/L | 0.121 | 0.161 | 0.133 |
| 0.2 | |||
| 0ng/L | 0.023 | 0.027 | 0 |
| 0.031 |
Precision
We measured random samples of E6611Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 909.76 | 35.34 | 3.88 |
| Intra-Assay | 2 | 18 | 583.93 | 22.73 | 3.89 |
| Intra-Assay | 3 | 18 | 408.31 | 16.08 | 3.94 |
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