Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E6632Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 3200ng/L | 1.873 | 1.849 | 1.814 |
| 1.824 | |||
| 1600ng/L | 0.946 | 1.001 | 0.966 |
| 1.057 | |||
| 800ng/L | 0.571 | 0.592 | 0.557 |
| 0.613 | |||
| 400ng/L | 0.311 | 0.296 | 0.261 |
| 0.281 | |||
| 200ng/L | 0.121 | 0.129 | 0.094 |
| 0.137 | |||
| 0ng/L | 0.033 | 0.035 | 0 |
| 0.037 |
Precision
We measured random samples of E6632Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 580.16 | 31.4 | 5.41 |
| Intra-Assay | 2 | 18 | 2602.38 | 84.97 | 3.27 |
| Intra-Assay | 3 | 18 | 1798.05 | 90.29 | 5.02 |
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